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#1. Carbodiimide Crosslinker Chemistry | Thermo Fisher Scientific
EDC couples NHS to carboxyls, forming an NHS ester that is considerably more stable than the O-acylisourea intermediate while allowing for efficient conjugation ...
#2. Different EDC/NHS Activation Mechanisms between PAA and ...
The EDC/NHS activation efficiency can be judged from the following three criteria, high yield of NHS-ester, low yield of byproducts such as ...
#3. 1-乙基-(3-二甲基氨基丙基)碳醯二亞胺 - Wikipedia
1-乙基-3-(3-二甲基氨基丙基)碳醯二亞胺(亦稱為EDC、EDAC或EDCI)是一種水溶性碳二亞胺,常製成鹽酸鹽之形式。其pH範圍通常在4.0-6.0。EDC常在伯胺製備醯氨時作為羧基 ...
#4. EDC/NHS activation mechanism of polymethacrylic acid
... were prepared on porous silicon for further investigation of the EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide) activation ...
#5. EDC reacts with a carboxylic-acid group on FEP sheet...
2), yielding a conjugate of the two molecules joined by a stable amide bond. The addition of sulfo-NHS stabilizes the amine-reactive intermediate by converting ...
#6. High Efficiency & Stability Protein CrossLinking with EDC & NHS
For higher coupling efficiency and more stable amine-reactive intermediates, EDC crosslinking protocols often include N-hydroxysuccinimide (NHS) ...
#7. Characterization of covalent crosslinking strategies for ...
The initial EDC/NHS reaction is performed at pH 4.5–7.5. Immediately afterwards, the pH of the reaction mixture is raised to approx. 8.0 prior ...
#8. Preparation and properties of EDC/NHS mediated ...
1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), a water-soluble, non-cytotoxic, and biocompatible carbodiimide offers a safe and effective ...
#9. Microsphere Coupling – Two-step EDC/Sulfo NHS Covalent ...
We present here a general two-step covalent coupling protocol for conjugation of proteins to Estapor® carboxyl- modified microspheres using EDC and Sulfo-NHS.
#10. Amine coupling through EDC/NHS: a practical approach
Surface plasmon resonance (SPR) is one of the leading tools in biomedical research. The challenge in its use is the controlled positioning of one of the ...
#11. Site-directed immobilization of antibody using EDC-NHS ...
Site-directed immobilization of antibody using EDC-NHS-activated protein A on a bimetallic-based surface plasmon resonance chip.
#12. The Importance of Reaction Conditions on the Chemical ...
Keywords: acylation reaction; chitosan derivatives; EDC/NHS coupling system; fatty acids; spectroscopic methods. 1. Introduction.
#13. EDC NHS | Sigma-Aldrich
Gonadotropin (hCG)'. emdmillipore.com/estapor 3. Application Note: 'Microsphere Coupling, Two-step EDC/Sulfo NHS Covalent Coupling Procedure for Estapor® ...
#14. Electrochemical impedance and spectroscopy study of the ...
EDC /NHS activation of the carboxyl groups on poly(ε-caprolactone)/poly(m-anthranilic acid) nanofibers. Z. Guler1, A. S. Sarac1,2,3*.
#15. Covalent Conjugation of Antibody to Gold Nanoparticles for ...
EDC & Sulfo-NHS – the critical reagents for the covalent coupling process. EDC is used to activate the carboxylic acid group on nanoparticles. Sulfo-NHS is ...
#16. eDC and eDC Gold Information - NHS Supply Chain
eDC Gold is your inventory management platform designed to manage consignment, high value and critical care products. Providing you with inventory visibility ...
#17. Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide ...
Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for ...
#18. Sulfo-NHS (sulfo-N-hydroxysuccinimide), Carboxylate-primary ...
Overview · Product name. Sulfo-NHS (sulfo-N-hydroxysuccinimide), Carboxylate-primary amine chemical modification reagent · Description. Carboxylate-primary amine ...
#19. Warning about EDC - BioNavis
This warning concerns you, if you use EDC-NHS activation chemistry for immobilization of your ligands onto sensors and if you source the EDC directly from ...
#20. The process of EDC-NHS cross-linking of ... - AIP Publishing
The process of EDC-NHS cross-linking of reconstituted collagen fibres increases collagen fibrillar order and alignment. APL Materials 3, 014902 (2015); ...
#21. The effect of different cross-linking conditions of EDC/NHS on ...
The porous EDC/NHS-crosslinked scaffolds were obtained through a two-step freeze-drying process. To determine the optimal crosslinking ...
#22. NHS / sulfo-NHS - INTERCHIM
protein modification: conversion of carboxyl groups to amine-reactive NHS esters. Largely used as activator to mediate. EDC reaction on carboxyls.
#23. EDC meeting papers - NHS England
EDC meeting papers. View the minutes, agendas and papers of previous Equality and Diversity Council (EDC) meetings: 2019. 22 October.
#24. Abbreviation / Long Form : EDC/NHS / ethyl ... - Allie
A Search Service for Abbreviation / Long Form. □ Abbreviation / Long Form : EDC/NHS / ethyl (dimethylamino propyl) carbodiimide/N-hydroxysuccinimide ...
#25. Bioconjugate Techniques - 第 263 頁 - Google 圖書結果
Sulfo -NHS. The water soluble carbodiimide EDC may be used to form active ester functionalities with car- boxylate groups using the water soluble compound ...
#26. Handbook of Surface Plasmon Resonance
The aminolysis of (sulfo-)NHS esters occurs smoothly at slightly alkaline pH, ... a short pulse of low concentration EDC/NHS over the surface-bound protein ...
#27. Controlling Coupling Reaction of EDC and NHS for ...
Collagen gel was prepared by using EDC and NHS as coupling agents. Ethanol did not denaturate the helical structure of the collagen and prevented the hydrolysis ...
#28. Instructions for the use of (PEG)n-Amine Reagents
coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulfo-NHS. Typical pegylation reagents contain heterogeneous mixtures of ...
#29. 利用自組裝單層膜技術製造奈米鑽石陣列晶片與其應用
-COOH,再加入EDC/NHS 可以與含氨基-NH2 的山葵過氧酵素(HRP)反應形. 成醯胺鍵(Amide bond) [17]。 訊號的偵測,是利用生物分子反應作用後,改變電荷變化[18-19],或.
#30. Immunodox®-Amine (PEGylated) - Encapsula NanoSciences
EDC /sulfo-NHS coupling reactions are highly selective and highly efficient, and the biological activity of the protein or peptide is preserved. Two-step ...
#31. Influence of EDC/NHS coupling chemistry on ... - NASA/ADS
The toxicity of ZnO nanoparticles (NPs) is a growing concern due to its increasing use in several products including sunscreens, paints, pigments and ...
#32. Edc Nhs Solution (Biacore) | Bioz | Ratings For Life-Science ...
Edc Nhs Solution, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.
#33. Anti-EGFR-EDC/NHS-Dox ADC - Creative Biolabs
This ADC product is composed of an anti-EGFR antibody conjugated via EDC/NHS linker to Dox (cetuximab-EDC/NHS-Dox). It has demonstrated a response in ...
#34. Enhanced physicochemical properties of collagen by using EDC
The present study investigated the collagen scaffolds with and without 1-ethyl-3-(3-dimethyl amino- propyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS)- ...
#35. EDC/NHS cross-linked collagen foams as scaffolds for ...
roxy succinimide (EDC/NHS) cross-linking to yield longer lasting, porous scaffolds with a thickness similar to that of native cornea (500 µm).
#36. EDC Plus Sulfo-NHS - iGEM 2017
EDC Plus Sulfo-NHS. 1. Dissolve 1mg L-lysine in 1ml of activation buffer (0.1M MES, 0.5M NaCl,. pH6.0), at a concentration of 1mg/ml.
#37. In situ study of EDC/NHS immobilization on ... - Research NCKU
In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy ( ...
#38. 中華民國第56 屆中小學科學展覽會作品說明書佳作
圖二EDC 與NHS 的反應機構. Page 6. 5. 二、實驗一:合成奈米金粒子. (一) 目的:合成出特定半徑之奈米金粒子,我們希望合成出13nm 的奈米金粒子。 (二) 步驟:. 1. 以王水 ...
#39. Enhancing conjugation rate of antibodies to carboxylates
A typical reaction for coupling the carboxylic acid groups of SAMs with the amine residue of antibodies in the presence of EDC/NHS is depicted in Figure ...
#40. EDC / NHS不同交联条件对II型胶原蛋白支架的影响
The porous EDC/NHS-crosslinked scaffolds were obtained through a two-step freeze-drying process. To determine the optimal crosslinking condition, ...
#41. Preparation and biocompatibility of EDC-NHS crosslinked ...
Preparation and biocompatibility of EDC-NHS crosslinked chitosan-polyethyleneoxide-silk fibroin electrospun nanofibers[J].
#42. EDC/NHS problem - Forum - SPR-Pages
Hi, We used gold-covered glass chip and we incubated in MUA-11 in overnight. While we activated the chip surface with EDC/NHS, ...
#43. Sulfo-NHS-Biotin - APExBIO
Sulfo -NHS-Biotin (N-Hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. The most common amine-reactive biotinylation reagents can be ...
#44. EDC/NHS交联对胶原物理化学性能的影响
Influence of EDC/NHS Crosslinking on Physicochemical Properties of Collagen ... 二甲基氨丙基)-碳化二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)交联剂对胶原进行交联, ...
#45. Enhanced physicochemical properties of collagen by using EDC
The present study investigated the collagen scaffolds with and without 1-ethyl-3-(3-dimethyl amino- propyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS)- ...
#46. The process of EDC-NHS cross-linking of ... - OSTI.GOV
We describe the production of collagen fibre bundles through a multi-strand, semi-continuous extrusion process. Cross-linking using an EDC (1- ...
#47. Description Storage Introductions Procedure for Using EDC ...
EDC is a carboxyl and amine-reactive zero-length crosslinker. ... Procedure for Two-step Coupling of Proteins Using EDC and NHS or Sulfo-NHS.
#48. EDC/NHS - List of Frontiers' open access articles
This page contains Frontiers open-access articles about EDC/NHS.
#49. e1335d75e49db9c0fb2d26d73b...
EDC along with NHS or sulfoNHS. We have employed a wide range of antibody crosslinking strategies for immunodiagnostic applications.
#50. Oliver Hardick, Coupling Protocols - UCL
List of protocols. Aldehyde Functionalisation CA - DEAE Derivatisation CA - Epichlorohydrin Activation EDC NHS Activation ...
#51. γ辐射和EDC/NHS改性对胶原壳聚糖支架性能的影响
Effects of γ-irradiation and EDC/NHS modification on property of collagen-chitosan scaffolds[J]. Journal of Materials Engineering, 2020, ...
#52. Covalent Conjugation of Proteins to Carboxylated Gold ...
EDC and NHS "activate” the carboxyl groups on the particle surface to form an intermediate that can subsequently react with primary amine groups on the specific ...
#53. Coupling of substances containing a primary amine to ...
Lowest NHS ratio . ... EDC. 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide. HA. Hyaluronan. HPLC. High performance liquid chromatography.
#54. DBCO-Sulfo-NHS ester (sodium salt) - Jena Bioscience
The DBCO-sulfo-NHS ester is a water soluble, amine-reactive reagent for the incorporation of a DBCO moiety to an available amine functionality.
#55. Characterization of covalent crosslinking strategies for ...
EDC and EDC/NHS reactions have been extensively used before, their reaction efficiencies have only been quantified indirectly [9] or in semiquantative FTIR.
#56. LEGENDplex™ Carboxyl Bead Conjugation Protocol
8. EDC solution (1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride). 9. Sulfo-NHS (N-hydroxysulfosuccinimide). 10. Blocking Buffer.
#57. Amine Coupling Kit - VWR
Ligands are coupled via primary amine groups to the sensor surface for subsequent analysis. Reagent preparation. Action. Step. Dissolve the EDC and NHS by ...
#58. A systematic analysis of DMTMM vs EDC/NHS for ligation of ...
The activation of carboxyl groups with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (EDC/NHS) for amide formation is ...
#59. Activity analysis of the carbodiimide-mediated amine coupling ...
dimethylaminopropyl]carbodiimide (EDC)/NHS activation details of carboxy-terminated groups on a silicon surface [19,28–30]. Their.
#60. EDC/NHS电化学最常用的反应路径之一- 肖鑫鑫的博文
EDC /NHS 作为Zero-length crosslinker,是用来枝接氨基和羧基是在电化学领域修饰电极常用的一对试剂,因为没有在链上引入多余的碳链等而广为应用。
#61. Cysteine immobilisation on the polyethylene terephthalate ...
The 2-step crosslinking mechanism uses EDC/NHS for activating the carboxyl groups initially on the PET-[COOH] to form a stable intermediate, ...
#62. 6-Azidohexanoic Acid Sulfo-NHS Ester | Click Chemistry Tools
6-Azidohexanoic Acid Sulfo-NHS Ester is water-soluble reagent that enables simple and efficient labeling of antibodies, proteins and any other primary ...
#63. EDC-HCl (EDAC) Crosslinker Protocol - NET
Facilitated through a reactive carbodiimide (EDC-HCl) and Sulfo-NHS as a catalyst, this coupling procedure is a highly efficient choice for crosslinking ...
#64. Amine coupling of ligand to Biacore sensor chips - Cytiva
Solutions required for immobilization of ligands by amine coupling. EDC. 0.4 M of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide in Milli-Q™ water. NHS.
#65. A-systematic-analysis-of-DMTMM-vs-EDC_NHS-for-ligation-of ...
compare 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) to EDC/NHS activation chemistry for HA ligation using an array of ...
#66. Hyaluronic acid/EDC/NHS-crosslinked green electrospun silk ...
Hyaluronic acid/EDC/NHS-crosslinked green electrospun silk fibroin nanofibrous scaffolds for tissue engineering [2016]. Yang, Xingxing; Wang, Xiaoyun; Yu, ...
#67. ligand immobilization using - Click Coupling - XanTec ...
Besides EDC/NHS coupling, affinity based ligand immobilization via streptavidin–biotin linkage has become one of the most popular immobilization methods for ...
#68. The effect of the carbodiimide cross-linker on the structural and
Abbreviations: CO, collagen; CS, chondroitin sulfate; EDC, 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride; NHS, N-hydroxy ...
#69. Re: [求救] 請問NHS EDC的用途及溶劑- 看板Biotech
好久沒有做實驗了,憑久遠的記憶試著幫你解答一下,如果有誤請大家鞭小力一點.. 1.EDC和NHS是活化COOH基用的,EDC先和COOH基反應,然後再由NHS和這個 ...
#70. Surface Functionalization by Amidation Reaction (NHS-ester ...
#71. Pierce Biotechnology - EDC - 细胞生物
amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not ...
#72. EDC-NHS Meaning | What Does EDC-NHS Stand For? - All ...
What does EDC-NHS abbreviation stand for? List of 1 best EDC-NHS meaning form based on popularity. Most common EDC-NHS abbreviation full form updated in ...
#73. Analytical Biotechnology - 第 6 頁 - Google 圖書結果
Protocol 2 EDC combined with sulfo - NHS Sulfo - NHS is able to increase the efficiency of EDC - mediated reactions by forming sulfo - NHS ester ...
#74. Worldwide Research Efforts in the Fighting Against Microbial ...
Reference wool 2. wool+EDC+NHS+pLys 3. wool+EDC+NHS 4. wool+pLys Extracted wool after treated with EtOH Figure 3: Zeta potentials (ζ) of wool samples.
#75. Soft Matter for Biomedical Applications - Google 圖書結果
... with catechol Succinylation EDC/NHS coupling Supramolecular interactions Click chemistry EDC/NHS for catechol coupling Bioadhesives137 Wound dressing134 ...
#76. 5_1부. 고체상 위의 고정화 방법 (immobilization method on ...
여기서 조력자들은 EDC, NHS 라는 제로링커 (zero-linker)들입니다. 이 친구들은 반응 중간에 잠시 개입했다가, 최종적으로는 떨어져 나가는 그런 ...
#77. Electrospun Biomaterials and Related Technologies
The synthetic and natural crosslinking agents that have been used are: genipin, glutaraldehyde, EDC and EDC with N-hydroxysulfosuccinimide (EDC-NHS).
#78. Protein-Functionalized Hairy Diamond Nanoparticles
... further decorated by bovine serum albumin through the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling procedure.
#79. 华南理工大学学报(自然科学版)
... 石蜡/膨胀石墨复合相变材料的结构与热性能 (4089); EDC/NHS 交联对胶原物理化学性能的影响 (3321); 超弹性膜的等轴拉伸试验方法及仿真 (3314).
#80. Lakeville North High School / Homepage - ISD194.org
Lakeville Area Schools joins the Inclusive Schools Network, EDC, and schools, districts, and communities around the world in declaring the week of December ...
#81. 氨基硅烷涂层载玻片的应用介绍_检测_分子 - 手机搜狐网
它与具有EDC/NHS激活的羧基的生物分子共价结合。 胺官能化载玻片的应用. 氨基硅烷涂层载玻片是印刷PCR和长寡核苷酸比较受欢迎的选择产品。
#82. 81912824.pdf - CORE
EDC /NHS, followed by reaction with an amine to yield an amide linkage. SCHEME 2. Activation of a carboxylic acid terminated monolayer using.
#83. Emergency Care Data Set (ECDS) - NHS Digital
It replaced Accident & Emergency Commissioning Data Set (CDS type 010) and was implemented through: ECDS (CDS 6.2.2 Type 011). ECDS allows NHS ...
#84. CINECA IRIS Institutional Research Information System
In a next step, DNA immobilization on the activated polymers was studied by using three different coupling systems, namely: EDC/NHS, dopamine, and tyrosine.
#85. Special Offers, Sales & Discounts - UKMCPro
Find a large range of Special Offers, Sales & Discounts on UK based UKMCPro.co.uk, your Military, Police and Outdoor Store ✓ Free Delivery ✓ Best Prices ...
#86. Introduction to EDCs | Endocrine Society
The guide draws from each organization's strengths to present a comprehensive picture of global EDC exposures and health risks. Endocrine Society authors ...
#87. 新晋院士:顾宁|细胞|纳米 - 网易
同时,通过在体系中引入EDC/NHS,促进了水凝胶-组织界面上酯酰胺共价键的形成,进一步增强了粘附力。这种通过纳米离子储库策略获得的双网络水凝胶可 ...
#88. Discussion: Introduce yourself here! • Global Health Trials
... a recent transition of how research is delivered in the NHS. ... CRO with a Cloud-based EDC solution based on open source ClinCapture.
#89. Medical Device News and Press Releases|Japan MDC, LLC
... and potentially an immediate scalable solution to NHS ... Acquires Endoscopy Development Company The addition of EDC to the HCG family provides ...
#90. Review to be launched into racial and gender bias in medical ...
He added: “One of the founding principles of our NHS is equality, and the possibility that a bias — even an inadvertent one — could lead to ...
#91. Health-Tracking T-Shirts - NewsBreak
... maximize its ability to be used in a variety of different scenarios. ELECTRONICS・ 1 DAY AGO. Picture for Multifunctional EDC Scalpels.
#92. Covid, un'Europa a due velocità: ecco perché quella ...
Da oggi il sistema sanitario (Nhs) inserisce nel suo passaporto vaccinale ... Mig, novità 2021 un'area dedicata alla cioccolateria. e.d.c. ...
#93. Best Omnia Podcasts (2021) - Player FM
... Amanda has also performed worldwide at ULTRA KOREA, EDC a . ... which supports innovation in all its forms within the NHS and Social Care.
#94. Budget boost for low-earners as minimum wage rises to £9.50 ...
Public sector pay was frozen during the pandemic last year except for NHS workers but Sunak could announce rises for workers such as ...
#95. Cameroun > Diplomatie parlementaire: Cavaye reçoit une ...
... rra bb aaa qbpk evc ch jg bqv bfdk igcb kkei edc lba bccc jc lame ... pf nej hcf bb bafa nhs ca ab ch gi cde cee adba hgh cbde jen bcff ...
#96. Bee Free Honee Net Worth 2020 | Thug Life Meme
... ia hp io bfe edc ic dlhm da bh cde sgft jh nf adcd bi umn ko cunc ... ici ba bac il aaaa qj edg dd nhs aa dc fcd jir chhf fhf jona ogpq ...
edc nhs 在 Re: [求救] 請問NHS EDC的用途及溶劑- 看板Biotech 的推薦與評價
※ 引述《coolmeow (誰能愛我100趴)》之銘言:
: 抱歉在這個版上問這好像很淺....
: 最近老師要作生物試片以測試實驗室自己架的光學機構 如...SPR 但尚無流道
: 我看了一些paper的步驟 但是實驗室實在沒有生醫基礎
: 所以有一些問題或許很蠢但是請各位包涵
: 1.請問NHS及EDC的在表面改質的步驟上到底有什麼樣的功能或目的?
: 2.NHS及EDC裡面都有CH"火巠"類 這樣算是有機物嗎? 所以他的溶劑是水還是乙醇呢?
: 3.我們使用的表面改質SAM是MHDA 有16個c的tail group 一端S 一端COOH基
: 請問這個東西真的只要"浸泡12小時"就可以接於Au表面嗎?
: 我對於這一點非常懷疑.........(有無需要考濾水溫或其他大氣壓力等因素)
: 4.我們使用生物分子為BSA 再請問BSA的溶劑"PBS"的成分有沒有所謂的標準
: 因為我們上了SIGMA買的成分是有PB 10mM + NaCl 138mM + KCl2.7mM
: 編號是P-3813 但是參考其他碩論或是paper 不是沒有詳細交代其過程成分
: 就是寫的太專業看不懂....囧. 所以我們先姑且買了這個來用
: 5.請問加入BSA時的PBS有沒有需要顧慮水溫或滅菌 有些paper好像說要體溫(37度)
: 但有些又沒有交代.....
: 6.有許多文章有提及tween啥的.... 請問這倒底是在幹嘛的呢
: 主要功能是......?
: 抱歉一下子問了一堆問題
: 拜託來個神人幫我解答吧 (跪求...)
好久沒有做實驗了,憑久遠的記憶試著幫你解答一下,如果有誤請大家鞭小力一點..
1.EDC和NHS是活化COOH基用的,EDC先和COOH基反應,然後再由NHS和這個基團反應,
最後變成 R-C=O -NHS的反應基團,接下來就可以和帶有 R2-NH2的基團反應,變更
穩定的 R-C=O -NH -R2 鍵結
2.NHS和EDC用水就可以溶解了
3.會啊,這就是所謂的Self assembly monolayer,可以去google一下相關原理,不過
如果我沒記錯,成不成功和水溫和壓力的因素不大,和碳鏈的長短和浸泡時間關係
比較大...
4.PBS就是phosphorate buffer saline,要選哪一種基本上和你未來的實驗設計比較
有關吧...
5.同4,和你實驗設計比較有關,不然隨便加一加也會溶解
6.Tween是一種介面活性劑,他是商品名,依照不同的特性有Tween-20,Tween-80...等
很多種類,主要是避免非特異性吸附用的...
希望對您有幫助,如果有誤也請其他神人補充改正一下...
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